Abstract: Pilot Study. Hand Hygiene Skin ATP Study; A unique comparison of Tap Water vs. Ozonated (O3) Water vs Antiseptic + O3 Water vs Antiseptic + Tap Water in regard to Adenosine Triphosphate (ATP) Reduction on the Skin, as a measure of biologic material reduction.
Alvaro Liceaga, M.D., Elvin Mercado, C.S.T., Lisa Liceaga B.S. , Mary Narcarcoti R.N.MSHA.
Clean hygienic hands are an important factor in preventing the spread and in the reduction of the incidence of nosocomial infections in the healthcare setting. Despite best efforts to clean hands of bacteria and other organisms, handwashing with soap is only partially effective in this regard. Additionally, a number of sanitizing chemicals have been used in the medical setting, but some are now banned in the USA. This pilot study was undertaken to evaluate ozonated water generated through a Lenova Faucet System.
Water, when “Ozonated” (O3-H20), produces free radical oxygen molecules that react with the microbes and effectively kill them via oxidation and degeneration of organic compounds. The US EPA Office of Water wrote that ozone is one of the most potent and effective germicides (US EPA, 1999).
In this study, Bioload was measure d in ATP levels. The hands of the volunteers that were cleaned with soap and rinsed in tap water resulted in average of 85.76% reduction in ATP levels. The hands of the volunteers that were cleaned with Hibiclens antiseptic solution and rinsed in tap water resulted in an average of 94.2 % reduction in ATP levels. The hands of the volunteers that were cleaned with soap and rinse with ozonated water resulted in an average of 97.3% reduction in ATP levels. The hands of the volunteers that were cleaned with Hibiclens antiseptic solution and rinsed with ozonated water resulted in an average of 97.5% reduction in ATP levels. There was no recognizable evidence that any of the cleansing techniques caused any adverse effects to the skin tissue of the test subjects.
It is believed impossible to eliminate all ATP from skin cells and achieve a zero “100%” ATP result. The teaching of proper handwashing technique, and the enforcement and re-evaluation of healthcare workers’ methods of handwashing is of great importance. The addition of ozonated water to the handwashing regimen is recommended by the authors, as it has proven to be of value in reducing ATP levels on the skin following this preliminary study. Further studies are needed to issue additional recommendations.
Keywords: Ozone Water; Hygiene; Sanitizing; Oxidation; Medical; Surgery; Microorganisms; Bioload; ATP; Handwashing.
Document Type: Pilot Research Article
Affiliations: Alliance Surgery Center Inc. Fullerton, CA
Publication date: January 09, 2017